易必迪 ibidi 細胞骨架染料 LifeAct Adenoviral Vectors
貨 號：60121/60122/60124
ibidi 活細胞骨架染料LifeAct Adenoviral Vectors
LifeAct是一個來源于酵母菌蛋白的17個氨基酸組成的多肽，可以對活細胞或固定細胞的F-Actin染色。
與GFP-actin和其他的actin綁定蛋白相比，LifeAct的優勢在于無論體內還是體外都不影響actin動力、
作為一個新型actin marker，它獨特的特性使之成為今天好用的F-Actin marker。
ibidi熒光標記rAV-LifeAct轉導細胞之后，通過熒光marker tagGFP2或TagRFP使F-actin可視化。
通過病毒載體轉導，對于一些難轉染的細胞，如原代細胞和神經元細胞，效率也可以達到99%。
*Original LifeAct publication in Nature Methods:
Riedl J, et al. Lifeact – a versatile marker for the visualization of F-actin. Nature Methods 5, 605-607 (2008)
易必迪 ibidi 細胞骨架染料 LifeAct Adenoviral Vectors 應用：
1 . 細胞骨架結構
無毒性actin 可視化
熒光染色
2. 細胞骨架動力學研究
細胞生物進程中actin 動力學研究
活細胞成像和顯微觀察
易必迪 ibidi 細胞骨架染料 LifeAct Adenoviral Vectors 技術優勢
· 活細胞中F-Actin的可視化。得到細胞骨架和動力學的理想圖像。
· 無毒性，不會影響Actin的功能和動力學。
· 理想的信噪比。
· 可以用于難轉染的細胞，如原代細胞 。
能達到99%轉導效率。
易必迪 ibidi 細胞骨架染料 LifeAct Adenoviral Vectors 規格
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Concentration
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1 x 1010 IU / ml
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Storage
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-80 °C
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TagGFP2 (Exmax / Emmax)
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483 / 506 nm
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TagRFP (Exmax / Emmax)
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555 / 584 nm
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易必迪 ibidi 細胞骨架 HT-1080 LifeAct-TagGFP2 Stable Cell Line
貨 號：40101/40101
A stable LifeAct-TagGFP2 expressing human fibrosarcoma cell line
Long-term actin visualization - 25 passages guaranteed
Characteristics proven to be identical to the parental HT-1080 cell line
LifeAct technology guarantees no interference with cytoskeletal dynamics
易必迪 ibidi 細胞骨架染料 LifeAct Adenoviral Vectors Applications:
Live cell imaging of cytoskeletal organization and dynamics
Functional cell-based assays, such as chemotaxis and migration
Usable as a characterized control in your experiments
To create the stable LifeAct-TagGFP2 expressing cell line, human fibrosarcoma cells were stably transfected with a plasmid coding for
TagGFP2-labeled LifeAct .
The HT-1080 LifeAct-TagGFP2 cells present highly dynamic filamentous actin. As a result of stable LifeAct expression, perfect visualization
of filamentous actin with very low background fluorescence is achieved without any interference to cellular dynamics.
Moreover, full characterization of the cells revealed that they displayed identical behavior to wild type (wt) cells.
Due to the cell line’s fast growth and the absence of contact inhibition, it constitutes an ideal tumor model.
These characteristics combined with bright and specific F-actin staining lead to a powerful tool with versatile applications in live cell imaging and
high-content approaches.
Specifications:
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Cells/vial
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5 x 105
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Medium
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ibidi Freezing Medium
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Storage
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Liquid nitrogen
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Biosafety Level
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1
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Sterility
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Tested for yeast and bacteria
including mycoplasma
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Promoter
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CAG (modified chicken β-actin)
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Transgene
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LifeAct-TagGFP2*
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*For further information on TagGFP2, please refer to www.evrogen.com.
Origin information:
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Organism
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Homo sapiens (human)
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Source
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Connective tissue disease: fibrosarcoma
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Age
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35 years
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Gender
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Male
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Ethnicity
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Caucasian
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Growth properties
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Adherent
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Morphology
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Epithelial
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Technical Features:
Homogeneous cell pool
Assured cell line identity
Purity tested
Optimal transgene expression for microscopy assays
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