貨號
產品名稱
規格（個/盒）
80286
µ-Slide 2孔腔室載玻片，ibiTreat底部處理
15
80282
µ-Slide 2孔腔室載玻片，Collagen IV底部處理
80283
µ-Slide 2孔腔室載玻片，Fibronectin底部處理
80284
µ-Slide 2孔腔室載玻片，Poly-L-Lysine底部處理
80285
µ-Slide 2孔腔室載玻片，Poly-D-Lysine底部處
80281
µ-Slide 2孔腔室載玻片，無包被
80287
µ-Slide 2孔腔室載玻片，玻璃底
μ兩孔培養載玻片
用于**顯微鏡和**熒光的開放式μ兩孔培養載玻片
一體化小室，既可用于細胞培養，也可用于顯微鏡成像
使用高分辨率顯微鏡可以通過玻璃片底部進行樣品觀察
沒有蓋玻片，不會泄露，用于快速，簡單的**熒光。
只需少量細胞和試劑，降低了實驗成本
開放式載玻片，具有兩個獨立的培養孔
具備適用于**顯微鏡觀察的優良光學成像特質
兼容染色，固定
采用*佳細胞粘附的表面處理Ibitreat
生物兼容性塑料生產，無膠水，不泄露
孔數量
2
每孔生成面積
4.8 cm²
孔尺寸大小
21.2 x 23.3 x 9.3
每孔包被面積
7.5 cm²
每孔推薦體積
1500 µl
底部配套的蓋玻片
No. 1.5
帶蓋子高度
10.8 mm
底部
ibidi 標準底部
相差顯微鏡是*常用的，細胞培養中的透射光學技術。使用相差顯微鏡時，把兩個相位環彼此調節好是非常重要的。在開放式培養孔中，空氣-水交界面處的彎月牙就像一個棱鏡折射出光路。這個會誤校相位環，造成顯微鏡圖像的對比性較差。
使用µ- 2孔ph+培養載玻片減少了彎月牙，無論孔在哪個位置，整個光學系統都是平行的
µ-Slide 2 well
µ-Slide 2 well Ph+
The µ-Slide 2 well Ph+ (Phase Contrast +) is designed for excellent optical quality, especially for cell culture when normal phase contrast microscopy is being used. Opposed to the classic µ-Slide 2 well, the Ph+ version provides a special intermediate plate in the center of the well. This plate flattens the meniscus that disturbs the phase contrast effect in normal open wells. Openings near the corners provide access to the wells for easy filling and aspiration of liquids.
This innovative technique supports meniscus-free phase contrast microscopy in a very convenient manner.
Cross Section Through a Well of the µ-Slide 2 well Ph+ with a Transmitted Light Beam Path
The illustration on the left shows the perturbing effect of a meniscus. Light is refracted on the air-water-interface, leading to poor contrast in microscopy. Only the small center part exhibits satisfying phase contrast.
Working with the µ-Slide 2 well Ph+ diminishes the meniscus and increases the area of nicely contrasted cells. This nice contrast is due to the parallel beam path that is created by the plate.
