易必迪 ibidi 腺病毒提纯试剂盒ibiPureTM Adeno Adenovirus Purification Kit 60681 60682
货 号：60681
ibiPureTM Adeno Adenovirus Purification Kit
A kit for chromatography-based adenovirus purification from a cell pellet
Fast preparation in only 20 minutes
Just 4, easy-to-perform steps
Efficient purification with significantly high yields
(109 – 1010 of infectious units (IU) from a single 15 cm dish)
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Cat. No.
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Description
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Pcs./Box
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RMB
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60681
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ibiPure Adeno Adenovirus Purification Kit, 12 preps
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1
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￥ 7830
|
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60682
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ibiPure Adeno Adenovirus Purification Kit, 24 preps
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1
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￥ 11682
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ibiPureTM Adeno Adenovirus Purification Kit
Applications:
· Adenovirus purification, after amplification in HEK293 cells
NOTE:
Please note that you will be working with samples containing an
infectious virus. Follow the recommended NIH guidelines for all
materials containing BSL–2 organisms.
ibiPureTM Adeno Adenovirus Purification Kit
Technical Features:
· Spin-column-based adenovirus purification, directly from cell pellet
· Anion exchange membrane with extraordinarily high binding
capacity for adenoviruses
ibiPureTM Adeno Adenovirus Purification Kit
Specifications:
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Kit Components
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Equilibration Buffer 1-3, Loading Buffer,
Wash Buffer, Elution Buffer, Glycerol,
Spin Columns, Collection Tubes
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Expected Viral Titer
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109 - 5x1010 IU/ml*
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Final Volume of Purified Adenovirus
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500 µl
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Viral Serotype
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The kit is designed for the purification of
adenovirus serotype 5. The user must
test all other serotypes.
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Storage
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Room temperature
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* Titer was determined using the immunocytochemical detection of
adenovirus hexon protein in infected cells.
Recombinant adenoviruses are tools that are widely used in research,
and also in therapeutic applications. Due to their broad host tropism,
they are ideal vectors for transferring genes into the majority of
mammalian cells. Adenoviral vectors are the perfect tools for the
high-level expression of recombinant proteins (e.g., rAV-LifeAct Adenoviral Vectors ), and they also mediate very efficient
gene knockdowns. Adenoviral vectors are episomal vectors that do
not integrate into the host genome.
The production and amplification of adenoviral vectors occurs in producer
cell lines, such as HEK 293. From these cells, adenoviruses are released
by a freeze/thaw treatment, or by using other cell lysis methods.
Therefore, non-purified adenovirus preparations will contain cellular
and medium-derived proteins and other components that can negatively
affect transduced cells in vivo and in vitro. They can also reduce
stability during long-term storage.
Significantly Higher Virus Yields, Compared to the Industry Standard
Recombinant adenoviruses are tools that are widely used in research,
and also in therapeutic applications. Due to their broad host tropism,
they are ideal vectors for transferring genes into the majority of
mammalian cells. Adenoviral vectors are the perfect tools for the
high-level expression of recombinant proteins (e.g., rAV-LifeAct Adenoviral Vectors ), and they also mediate very efficient
gene knockdowns. Adenoviral vectors are episomal vectors that do not
integrate into the host genome.
The production and amplification of adenoviral vectors occurs in producer
cell lines, such as HEK 293. From these cells, adenoviruses are released
by a freeze/thaw treatment, or by using other cell lysis methods.
Therefore, non-purified adenovirus preparations will contain cellular
and medium-derived proteins and other components that can negatively
affect transduced cells in vivo and in vitro. They can also reduce
stability during long-term storage.
Consequently, working with purified adenovirus preparations is highly
recommended. For this reason, the ibiPure™ Adeno Adenovirus Purification Kit was developed. It is a robust and
reliable, spin-column-based system for quick and convenient adenoviral
purifications, with high recovery rates.
The purification protocol is almost as simple as that of most plasmid
purification systems. Just equilibrate your column, load your sample,
wash, and then elute your purified virus.
Bind – Wash – Elute:
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