易必迪 ibidi 细胞骨架染料 LifeAct Adenoviral Vectors
货 号：60121/60122/60124
ibidi 活细胞骨架染料LifeAct Adenoviral Vectors
LifeAct是一个来源于酵母菌蛋白的17个氨基酸组成的多肽，可以对活细胞或固定细胞的F-Actin染色。
与GFP-actin和其他的actin绑定蛋白相比，LifeAct的优势在于无论体内还是体外都不影响actin动力、
作为一个新型actin marker，它独特的特性使之成为今天好用的F-Actin marker。
ibidi荧光标记rAV-LifeAct转导细胞之后，通过荧光marker tagGFP2或TagRFP使F-actin可视化。
通过病毒载体转导，对于一些难转染的细胞，如原代细胞和神经元细胞，效率也可以达到99%。
*Original LifeAct publication in Nature Methods:
Riedl J, et al. Lifeact – a versatile marker for the visualization of F-actin. Nature Methods 5, 605-607 (2008)
易必迪 ibidi 细胞骨架染料 LifeAct Adenoviral Vectors 应用：
1 . 细胞骨架结构
无毒性actin 可视化
荧光染色
2. 细胞骨架动力学研究
细胞生物进程中actin 动力学研究
活细胞成像和显微观察
易必迪 ibidi 细胞骨架染料 LifeAct Adenoviral Vectors 技术优势
· 活细胞中F-Actin的可视化。得到细胞骨架和动力学的理想图像。
· 无毒性，不会影响Actin的功能和动力学。
· 理想的信噪比。
· 可以用于难转染的细胞，如原代细胞 。
能达到99%转导效率。
易必迪 ibidi 细胞骨架染料 LifeAct Adenoviral Vectors 规格
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Concentration
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1 x 1010 IU / ml
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Storage
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-80 °C
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TagGFP2 (Exmax / Emmax)
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483 / 506 nm
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TagRFP (Exmax / Emmax)
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555 / 584 nm
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易必迪 ibidi 细胞骨架 HT-1080 LifeAct-TagGFP2 Stable Cell Line
货 号：40101/40101
A stable LifeAct-TagGFP2 expressing human fibrosarcoma cell line
Long-term actin visualization - 25 passages guaranteed
Characteristics proven to be identical to the parental HT-1080 cell line
LifeAct technology guarantees no interference with cytoskeletal dynamics
易必迪 ibidi 细胞骨架染料 LifeAct Adenoviral Vectors Applications:
Live cell imaging of cytoskeletal organization and dynamics
Functional cell-based assays, such as chemotaxis and migration
Usable as a characterized control in your experiments
To create the stable LifeAct-TagGFP2 expressing cell line, human fibrosarcoma cells were stably transfected with a plasmid coding for
TagGFP2-labeled LifeAct .
The HT-1080 LifeAct-TagGFP2 cells present highly dynamic filamentous actin. As a result of stable LifeAct expression, perfect visualization
of filamentous actin with very low background fluorescence is achieved without any interference to cellular dynamics.
Moreover, full characterization of the cells revealed that they displayed identical behavior to wild type (wt) cells.
Due to the cell line’s fast growth and the absence of contact inhibition, it constitutes an ideal tumor model.
These characteristics combined with bright and specific F-actin staining lead to a powerful tool with versatile applications in live cell imaging and
high-content approaches.
Specifications:
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Cells/vial
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5 x 105
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Medium
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ibidi Freezing Medium
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Storage
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Liquid nitrogen
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Biosafety Level
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1
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Sterility
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Tested for yeast and bacteria
including mycoplasma
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Promoter
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CAG (modified chicken β-actin)
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Transgene
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LifeAct-TagGFP2*
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*For further information on TagGFP2, please refer to www.evrogen.com.
Origin information:
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Organism
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Homo sapiens (human)
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Source
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Connective tissue disease: fibrosarcoma
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Age
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35 years
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Gender
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Male
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Ethnicity
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Caucasian
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Growth properties
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Adherent
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Morphology
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Epithelial
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Technical Features:
Homogeneous cell pool
Assured cell line identity
Purity tested
Optimal transgene expression for microscopy assays
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