货号
产品名称
规格（个/盒）
80286
µ-Slide 2孔腔室载玻片，ibiTreat底部处理
15
80282
µ-Slide 2孔腔室载玻片，Collagen IV底部处理
80283
µ-Slide 2孔腔室载玻片，Fibronectin底部处理
80284
µ-Slide 2孔腔室载玻片，Poly-L-Lysine底部处理
80285
µ-Slide 2孔腔室载玻片，Poly-D-Lysine底部处
80281
µ-Slide 2孔腔室载玻片，无包被
80287
µ-Slide 2孔腔室载玻片，玻璃底
μ两孔培养载玻片
用于**显微镜和**荧光的开放式μ两孔培养载玻片
一体化小室，既可用于细胞培养，也可用于显微镜成像
使用高分辨率显微镜可以通过玻璃片底部进行样品观察
没有盖玻片，不会泄露，用于快速，简单的**荧光。
只需少量细胞和试剂，降低了实验成本
开放式载玻片，具有两个独立的培养孔
具备适用于**显微镜观察的优良光学成像特质
兼容染色，固定
采用*佳细胞粘附的表面处理Ibitreat
生物兼容性塑料生产，无胶水，不泄露
孔数量
2
每孔生成面积
4.8 cm²
孔尺寸大小
21.2 x 23.3 x 9.3
每孔包被面积
7.5 cm²
每孔推荐体积
1500 µl
底部配套的盖玻片
No. 1.5
带盖子高度
10.8 mm
底部
ibidi 标准底部
相差显微镜是*常用的，细胞培养中的透射光学技术。使用相差显微镜时，把两个相位环彼此调节好是非常重要的。在开放式培养孔中，空气-水交界面处的弯月牙就像一个棱镜折射出光路。这个会误校相位环，造成显微镜图像的对比性较差。
使用µ- 2孔ph+培养载玻片减少了弯月牙，无论孔在哪个位置，整个光学系统都是平行的
µ-Slide 2 well
µ-Slide 2 well Ph+
The µ-Slide 2 well Ph+ (Phase Contrast +) is designed for excellent optical quality, especially for cell culture when normal phase contrast microscopy is being used. Opposed to the classic µ-Slide 2 well, the Ph+ version provides a special intermediate plate in the center of the well. This plate flattens the meniscus that disturbs the phase contrast effect in normal open wells. Openings near the corners provide access to the wells for easy filling and aspiration of liquids.
This innovative technique supports meniscus-free phase contrast microscopy in a very convenient manner.
Cross Section Through a Well of the µ-Slide 2 well Ph+ with a Transmitted Light Beam Path
The illustration on the left shows the perturbing effect of a meniscus. Light is refracted on the air-water-interface, leading to poor contrast in microscopy. Only the small center part exhibits satisfying phase contrast.
Working with the µ-Slide 2 well Ph+ diminishes the meniscus and increases the area of nicely contrasted cells. This nice contrast is due to the parallel beam path that is created by the plate.
