整个培养孔具有优良的相差-没有弯月面现象一体化小室，既可用于细胞培养，也可用于显微镜成像使用高分辨率显微镜可以通过玻璃片底部进行样品观察没有盖玻片，不会泄露，用于快速，简单的**荧光。

产品描述

货号	产品名称	规格（个/盒）
80446	µ-Slide4孔Ph+腔室载玻片，ibiTreat底部处理	15
80442	µ-Slide 4孔Ph+腔室载玻片，Collagen IV底部处理	15
80443	µ-Slide 4孔Ph+腔室载玻片，Fibronectin底部处理	15
80444	µ-Slide 4孔Ph+腔室载玻片，Poly-L-Lysine底部处理	15
80445	µ-Slide 4孔Ph+腔室载玻片，Poly-D-Lysine底部处理	15
80441	µ-Slide 4孔Ph+腔室载玻片，无包被	15
80447	µ-Slide 4孔Ph+腔室载玻片，玻璃底	15

μ培养载玻片 4孔^Ph+

Excellent Phase Contrast in the µ-Slide 4 well^Ph+

一个开放的四孔培养载玻片，带有一个特殊中间板，非常适用于相差和**的荧光显微镜

整个培养孔具有优良的相差-没有弯月面现象

一体化小室，既可用于细胞培养，也可用于显微镜成像

使用高分辨率显微镜可以通过玻璃片底部进行样品观察

没有盖玻片，不会泄露，用于快速，简单的**荧光。

技术特点

开放式，两个独立的培养孔载玻片

每个培养孔上的中间板可以避免弯液面形成

从两侧缝隙很容易加液，无气泡形成，

适用于**显微镜的优良光学成像特质

兼容染色，固定

*佳细胞粘附的表面Ibitreat

生物兼容性塑料生产，无胶水，不泄露

技术指标

孔数量	4	带盖子高度	10.8 mm
孔尺寸大小	21.2 x 11.0 x 3.0	每孔生成面积	2.2 cm²
每孔体积	700 µl	每孔生成面积	5.9 cm²
底部ibidi 标准底部

Phase contrast microscopy is the most commonly used, transmitted light technique in cell culture. When working with phase contrast microscopy, it is crucial to have the two phase rings adjusted to each other. In open wells, the meniscus at the air-water-interphase works like a lens that refracts the beam path. This miscalibrates the phase rings, leading to poor contrast in the microscopic image.

U-slide 4 well U-slide 4 wellPh+

Working with the µ-Slide 4 well Ph+ diminishes the meniscus, so that the whole optical system is aligned, no matter which position of the well is being imaged.

Cross Section Through a Well of the µ-Slide 4 well Ph+ with a Transmitted Light Beam Path

The illustration on the left shows the perturbing effect of a meniscus. Light is refracted on the air-water-interface, leading to poor contrast in microscopy. Only the small center part exhibits satisfying phase contrast.

Working with the µ-Slide 4 well Ph+ diminishes the meniscus and increases the area of nicely contrasted cells. This nice contrast is due to the parallel beam path that is created by the plate.

Comparison Ph+ Well versus Standard Well:
No Meniscus Effect in Ph+ Well